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Research Highlights

Functional cooperation of the glycine synthase-reductase and Wood–Ljungdahl pathways for autotrophic growth of Clostridium drakei
Number
Journal
Proceedings of the National Academy of Sciences
Year of publication
2020
Despite sharing the first four reactions, coutilization of the Wood–Ljungdahl pathway (WLP) with the glycine synthase-reductase pathway (GSRP) and reductive glycine pathway (RGP) to fix C1 compounds has remained unknown. In this study, using Clostridium drakei, we elucidated the role of the GSRP and RGP in the presence of the WLP, via a genome-scale metabolic model, RNA-seq, 13C isotope-based metabolite-tracing experiments, biochemical assays, and heterologous expression. Overall, the data suggested the pathways are functional under autotrophic conditions. Along with the WLP, GSRP and RGP convert CO2 to glycine and then to acetyl-phosphate and serine, which then obtain ATP by producing acetate and operate with limited reducing power. This is a unique coutilization of the pathways under autotrophic conditions in acetogens.

Systems and Synthetic Biology Lab. Department of Biological Sciences, Korea Advanced Institute of Science and Technology(KAIST).
291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea
Tel: 042)350-2660 / Email: bcho@kaist.ac.kr

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