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Comparative Primary Metabolic and Lipidomic Profiling of Freshwater and Marine Synechocystis Strains Using by GC-MS and NanoESI-MS Analyses

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Abstract

Synechocystis strains are considered as valuable resources for production of useful metabolites. Comparative primary metabolic and lipidomic profiling of freshwater and marine Synechocystis strains is needed to understand the physiological characteristics of each strain at molecular level. In this study, we performed comparative primary metabolic and lipidomic profiling of freshwater Synechocystis sp. PCC 6803 (hereafter Synechocystis 6803) and marine Synechocystis sp. PCC 7338 (hereafter Synechocystis 7338) by using gas chromatography-mass spectrometry and nano electrospray ionization-mass spectrometry. Neophytadiene, phytol, monogalactosyldiacylglycerol (MGDG), and digalactosyldiacylglycerol (DGDG) were significantly higher in Synechocystis 6803. Levels of alanine, aspartic acid, valine, linoleic acid, linolenic acid, oleic acid, plamitic acid, stearic acid, glucosylglycerol, sucrose, phosphatidylglycerol (PG), and diacylglyceryltrimethylhomoserine (DGTS) (only detected in Synechocystis 7338) were significantly higher in Synechocystis 7338. Our results provide basic information to choose Synechocystis strains for various industrial usages, and also provide fundamental information on the future development of novel Synechocystis strains to produce high levels of specific metabolites by metabolic pathway modulation.

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Acknowledgments

This work was supported by the Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (No. NRF-2016M1A5A1027464), National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) [NRF-2015R1A5A1008958], and by the Chung-Ang University Graduate Research Scholarship in 2018. The authors declare no conflict of interest. Neither ethical approval nor informed consent was required for this study.

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Authors and Affiliations

  1. College of Pharmacy, Chung-Ang University, Seoul, 06974, Korea

    YuJin Noh, Hwanhui Lee & Hyung-Kyoon Choi

  2. Department of Biological Engineering, Inha University, Incheon, 22212, Korea

    Seong-Joo Hong & Choul-Gyun Lee

  3. College of Pharmacy, Gachon University, Incheon, 13120, Korea

    Hookeun Lee

  4. Department of Biological Sciences, KAIST, Daejeon, 34141, Korea

    Byung-Kwan Cho

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Comparative Primary Metabolic and Lipidomic Profiling of Freshwater and Marine Synechocystis Strains Using by GC-MS and NanoESI-MS Analyses

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Noh, Y., Lee, H., Hong, SJ. et al. Comparative Primary Metabolic and Lipidomic Profiling of Freshwater and Marine Synechocystis Strains Using by GC-MS and NanoESI-MS Analyses. Biotechnol Bioproc E 25, 308–319 (2020). https://doi.org/10.1007/s12257-019-0432-8

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